Early Stage Researchers (ESR) position on CellEurope (EC FP7 ITN) project available at:

Seventh Framework Programme

Department of Molecular Biology and Cell Pathology

Third Faculty of Medicine, Charles University Prague

Ruska 87

100 00 Prague 10

Czech Republic

contact: Prof. Ilona Hromadnikova

Admission criteria

Research experience no more than 4 years
No PhD
Duration of appointment:36 months; the contract starts at latest from 1^st April 2013
Researchers must not have resided or carried out their main activity (work, studies, etc) in the country of their host organisation for more than 12 months in the 3 years immediately prior to the date of selection by the host institution. Short stays such as holidays are not taken into account.

CELLEUROPE Researcher Description

Fellow No	6
Name	ER6
Project Title	GvL effects of NK cells against human leukaemia cell lines - in vitro and in vivo studies
Skills/Experience	cell cultures, flow cytometry, ELISPOT, 51 Cr Release Assay, statistical analysis (STATISTICA program)
Recruiting Participant (Host)	UPRAGUE
Duration	36 months
Mentor	Prof. Hromadnikova
Line Manager	TBC

Employment Details

Planned Start Date	M6
Planned End Date	M42
Category	ESR
Actual Start Date
Actual End Date
Type of Contract	UPRAGUE ref.
Eligibility Requirements	Nationality: no nationality restrictions Experience: <=4 years research experience (no PhD)

Allowances (€)

Living	2666.33€
Mobility	589.40€
Monthly Salary	TBC

Description of Work

Active in WP 3 - Objective: Investigate expression of NK cell ligands, NK cell receptors and function activity using human leukaemia blasts.

Tasks

6.1 Investigate the in vitro PBMN cell cytotoxicity against human leukaemia cell lines from patients with CML, ALL and AML during the onset and/or relapse of the disease showing high membrane-bound Hsp70 positivity.

6.2 Investigate the cell surface expression NK cell ligands on the leukaemia blasts using flow cytometry.

6.3 Investigate the cell surface expression of inhibitory and activating receptors on NK cells before and after stimulation with hsp70 derived peptide using flow cytometry.

6.4 Investigate the stimulation of T lymphocytes, NTK cells, DC and monocytes after the pre-incubation of PBMN cells with interleukins alone and in combination with hsp70 derived peptide using flow cytometry.

6.5 Correlate, in collaboration with Project 7 and Project 8, cell cytotoxicity with the cell surface expression of hsp70 and NK cell ligands on leukaemia blasts, the cell surface expression of inhibitory and activating receptors on NK cells and expression of activation markers on other mononuclear cells.